Structure and evolution of transplantation antigens: partial amino-acid sequences of H-2K and H-2D alloantigens.

Structure and evolution of transplantation antigens: partial amino-acid sequences of H-2K and H-2D alloantigens.

Techniques for the amino acid sequence analysis of subnanomole quantities of polypeptides have been applied to characterize beta2-microglobulin and transplantation antigens of the mouse isolated from spleen cells by indirect immunoprecipitation. Eleven residues were identified throughout the NH2-terminal 27 residues of the beta2-microglobulin; all were identical to residues seen at the correspo...

Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene

The Tla region located on the murine 17th chromosome controls several serologically defined cell surface antigens. These antigens, referred to as Qa-1-5 and TL, are expressed on a variety of hematopoeitic cell populations. In the present studies we have immunoprecipitated isotopically labeled Qa-2 and H-2 molecules from mitogen-stimulated B6 spleen cells. Sequential immunoprecipitation experime...

Stability of Surface H-2K, H-2D, and Peptide-Receptive H-2K on Splenocytes

Murine pancreatic beta-cells express H-2K and H-2D but not Ia antigens

Direct microcytotoxicity testing and absorption analyses were employed to determine whether H-2K, H-2D, and Ia antigens are present on murine islet of Langerhans cells. Products of the H-2K and H-2D loci were found on beta-cells from three different mouse strains, but I-region (Ia) antigens were not detected. The absence of Ia gene products from islet cells may be of importance in explaining th...

Selective Turnover and Shedding of H-2K and H-2D Antigens is Controlled by the Major Histocompatibility Complex